{"product_id":"9781071620007","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eKarim, Ashty S.; Jewett, Michael C.\u003c\/h2\u003e \u003cp\u003eThis detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and practical, \u003ci\u003eCell‐Free Gene Expression: Methods and Protocols\u003c\/i\u003e serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2023-01-07\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071620007\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-1998-8\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 435\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44806636994700,"sku":"9781071620007","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071620007.jpg?v=1772849550","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071620007","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}