{"product_id":"9781071618462","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eDworkin, Sebastian\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic\/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (\u003ci\u003ec. elegans\u003c\/i\u003e, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and \u003ci\u003ein vivo\u003c\/i\u003e cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using \u003ci\u003ein-situ \u003c\/i\u003ehybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR\/Cas9-mediated deletion. Written in the format of the highly successful \u003ci\u003eMethods in Molecular Biology \u003c\/i\u003eseries, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. \u003c\/p\u003eAuthoritative and cutting-edge, \u003ci\u003eCraniofacial Development: Methods and Protocols\u003c\/i\u003e aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. \u003cp\u003e\u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2021-12-16\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071618462\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-1847-9\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 313\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44806634963084,"sku":"9781071618462","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071618462.jpg?v=1772849453","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071618462","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}