{"product_id":"9781071617199","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eZhang, Ren\u003c\/h2\u003e \u003cp\u003eThis book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR\/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eComprehensive and authoritative, \u003ci\u003eEssential Genes and Genomes: Methods and Protocols\u003c\/i\u003e is an ideal guide for researchers attempting to strip genetics down to its fundamentals.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2021-10-29\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071617199\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-1720-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 434\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":46543422062732,"sku":"9781071617199","price":224.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071617199.jpg?v=1772849387","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071617199","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}