Methods in Pharmacology and Toxicology: In Vitro Methods and Protocols

Yan, Zhengyin; Caldwell, Gary W.

This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. 

Details

Published by: Humana

Publication Date: 2021-07-10

Format: Hardcover

ISBN-13: 9781071615416

DOI: 10.1007/978-1-0716-1542-3

Dimensions: 254cm x178cm

Pages: 379