Methods in Molecular Biology: Genetic Improvement Methods

Tripodi, Pasquale

This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs,  mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field.

Details

Published by: Humana

Publication Date: 2020-12-03

Format: Hardcover

ISBN-13: 9781071612002

DOI: 10.1007/978-1-0716-1201-9

Dimensions: 254cm x178cm

Pages: 280