{"product_id":"9781071607466","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eOrdóñez-Morán, Paloma\u003c\/h2\u003e \u003cp\u003eThis detailed book encapsulates the most up-to-date methods of the intestinal stem cell field and provides guidance on a variety of techniques for studying intestinal stem cells properties. Beginning with a section on \u003ci\u003ein vitro\u003c\/i\u003e techniques to study different aspects of the intestinal stem cell functions by innovative imaging and functional assays, the volume continues with chapters detailing the single-cell transcriptional profiling method, the isolation of intestinal crypts to generate and establish 3D organoids, as well as different animal models of gastrointestinal cancer and examples of the use of \u003ci\u003ein vivo\u003c\/i\u003e methods for studying intestinal tumor-initiating cells or cancer stem cells. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and state-of-the-art, \u003ci\u003eIntestinal Stem Cells: Methods and Protocols\u003c\/i\u003e aims to provide comprehensive and easy to follow protocols designed to be helpful to both seasoned researchers and newcomers to this dynamic field.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2020-07-24\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071607466\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-0747-3\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 350\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":46543185019020,"sku":"9781071607466","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071607466.jpg?v=1772848675","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9781071607466","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}