{"product_id":"9780471121343","title":"Culture of Immortalized Cells","description":"\u003ch3\u003eCulture of Specialized Cells\u003c\/h3\u003e\u003ch1\u003eCulture of Immortalized Cells\u003c\/h1\u003e\u003ch3\u003eR. Ian Freshney | Mary G. Freshney\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Cell Biology\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003eCulture of Specialized Cells\u003cbr\u003e \u003cbr\u003e Culture of Immortalized Cells\u003cbr\u003e \u003cbr\u003e Edited by R. Ian Freshney and Mary G. Freshney\u003cbr\u003e \u003cbr\u003e The ability to develop immortalized cells in vitro is a powerfultool for the biological investigator. Most cells can only bereproduced unchanged for a limited number of cell generationsbefore the initial cell lines die. The immortalization of cellsestablishes continuous cell lines, playing an important role in thestudy of the biology of cell growth, differentiation, andsenescence. It also increases our understanding of aberrations inproliferative and positional control in malignant cells.\u003cbr\u003e \u003cbr\u003e Culture of Immortalized Cells provides the most up-to-date in vitromethods and safety procedures for the immortalization of primarycultures of various cell types. Coedited by one of the leadingfigures in the field, R. Ian Freshney, author of Culture of AnimalCells, Third Edition, Culture of Immortalized Cells is the latestvolume in the critically acclaimed Culture of Specialized CellsSeries. Preceding volumes in the series include Culture ofEpithelial Cells and Culture of Hematopoietic Cells.\u003cbr\u003e \u003cbr\u003e In this comprehensive handbook, leading experts offer the mostcurrent methods for the immortalization of cells, as well asspecific guidelines for the immortalization of kidney, and thyroidepithelia, hepatocytes, fibroblasts, chondrocytes, lymphocytes,astrocytes, and human umbilical vein endothelium. Each method isaccompanied by:\u003cbr\u003e * Step-by-step protocols\u003cbr\u003e * Detailed instructions for reagent preparation\u003cbr\u003e * Current information on the latest specialized equipment andsupplies\u003cbr\u003e \u003cbr\u003e This concise bench-top manual is essential for any laboratoryinvolved in establishing continuous cell lines from primarycultures. Investigators, laboratory technical staff, and studentsin cell biology, immunology, hematology, cancer biology,toxicology, and molecular biology, will find this contemporaryguide indispensable to their research.\u003c\/div\u003e\u003cdiv\u003e  \u003cp\u003e\u003cstrong\u003eR. IAN FRESHNEY\u003c\/strong\u003e, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique. \u003c\/p\u003e\n\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e15 April 1996\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eWiley\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eWiley-Liss\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9780471121343\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003ePaperback \/ softback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePage Count: \u003c\/td\u003e\n\u003ctd\u003e416\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWeight (oz): \u003c\/td\u003e\n\u003ctd\u003e22.02\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":44315609858188,"sku":"9780471121343","price":203.36,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9780471121343.jpg?v=1780116534","url":"https:\/\/fh90cf-fv.myshopify.com\/products\/9780471121343","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}